Multiple Buds Formation from Ovules of Sandersonia aurantiaca Hook.

Abstract

In order to micropropagate Sandersonia aurantiaca efficiently, investigated the combination and concentration of plant growth regulators and L-proline. The adventitious buds formation from ovule in primary culture was observed at the medium containing NAA and BAP, and 0.27 μM NAA and 2.22 μM BAP promoted those formation. By the subculture on the medium containing 0.54 μM NAA and 11.1 μM BAP after culturing on the primary culture medium containing 0.27 μM NAA and 2.22 μM BAP, high frequency of adventitious buds and multiple buds formation was observed. The formation rate of adventitious buds and multiple buds 4.5 months after culturing were 69.6% and 34.8% respectively under darkness. Although the supplement of L-proline to medium did not promote embryogenesis in subculture, adventitious buds and multiple buds formation in subculture were promoted by supplement of L-proline, and those formation rates in 2.61mM L-proline were 91.3 and 43.5% respectively.