Abstract
Four-week-old Wistar rats fed a standard diet （18% protein）, a high protein diet （91%
protein） and a protein-free diet （0% protein） were reared for one week and examined the
induction of AST enzyme. As a result, the AST activity was 1.9 times higher in the high protein
diet than in the standard diet. 5.6-fold induction was observed when compared to protein-free
diet. Analysis of the protein pattern by SDS-PAGE analysis of the cytoplasmic fraction of the
liver of rats showed that a protein with a molecular weight of 44,000 corresponding to AST was
induced in rats with a high protein diet. When Western Blot was performed using an anti-AST
antibody, it was found that the protein having a molecular weight of 44,000 recognized by SDSPAGE
was AST.
