Abstract
Oxidative stress induced by fine particulate matter 2.5 (PM2.5) is a potential cause of adverse health effects due to production of reactive oxygen species (ROS). Currently, dithiothreitol (DTT) assay is mostly used in epidemiological studies for the measurement of oxidative potential (OP) of PM2.5. However, the number of applications is somewhat limited because the assay requires tedious procedures with care due to use of photodegradable reagents. Then, we have developed a simple and rapid methodology for the measurement the OP of PM2.5 by applying Flow Injection Analysis (FIA) to the DTT assay. This paper describes theoretical backgrounds of the FIA-DTT assay including concept of oxidative stress, and principle of DTT assay, and briefly demonstrates results of field measurement of OP of PM2.5 collected in outdoor and indoor air.
