Indoor Environment
Online ISSN : 2186-4322
Print ISSN : 1882-0395
ISSN-L : 1882-0395
The measurement of house dust mite allergen by several tools for determination and the comparison of their value
Shigeru TAKEDAYasuaki MORITakatsugu INADAYoshihiro SATOKouji ITAGAKI
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2008 Volume 11 Issue 1 Pages 1-9

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Abstract
We measured the levels of mite allergens in 32 house dust samples with 2 kinds of tools for ELISA reagents and 2 kinds of tools for quick determining kits at the same time and examined the difference in the measurement value, relationship between the obtained data, the performance of the quick determining kits. Allergen levels measured by In tool were 120-190,000ng per g of fine-dust at Der f 1, 0-15,600ng at Der p 1, 120-192, 400ng at Der 1, and 2-1,880ng at Der 2, respectively. As for ELISA reagents, the difference between two methods was large in the allergen amount of Der 1, and the allergen amount of Der 2 was low in both methods, so it is necessary to increase the detection performance of the tools. Moreover, as for the quick determining kits, the relationship of the data between the kits and the ELISA reagents was high, and the usefulness of the kits was confirmed as a mite pollution index in the room.
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© Society of Indoor Environment, Japan
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