The Journal of Silk Science and Technology of Japan
Online ISSN : 1881-1698
Print ISSN : 1880-8204
ISSN-L : 1880-8204
Optimization of pretreatment and LiBr concentration for dissolving whole cocoon silk proteins
Katsura KojimaMaria MurakamiYasushi Tamada
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2013 Volume 21 Pages 45-47

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Abstract

To optimize a method for dissolving whole silkworm cocoon proteins, we pretreated cocoons with 70% ethanol and different concentrations of lithium bromide (LiBr) in solubilizing solution. Pretreatment with 70% ethanol improved the dissolution of the proteins in 9 M LiBr solution. LiBr solution at concentrations of 7.5 to 9.5 M buffered with 0.1 M Tris·HCl at pH 9.0 could dissolve whole proteins efficiently. The efficiency of dissolution was the same among different cocoons.

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© 2013 by The Japanese Society of Silk Science and Technology
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