The Journal of Silk Science and Technology of Japan
Online ISSN : 1881-1698
Print ISSN : 1880-8204
ISSN-L : 1880-8204
Simple Spectrophotometric Analysis of Flavonoids from the Cocoon Shell of the Silkworm, Bombyx mori
Chikara HirayamaEiji Okada
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2014 Volume 22 Pages 65-70


The cocoon shells of several silkworm races contain flavonoids. We recently found that cocoon shells of Ryokuken races such as Daizo contain specific flavonoids that are almost non-existent in cocoon shells of Sasamayu races such as Pure Mysore. Usually, LC-MS systems are used to analyze flavonoids in cocoon shells. However, these systems require a great deal of time and effort to operate and maintain. Here, we confirmed that absorbance at a wavelength of 365 nm of aqueous methanolic extracts of the cocoons was well correlated with the total flavonoid content of the cocoons. The absorbance ratio (A420 nm: A365 nm) of the extracts was correlated with the percentage of Ryokuken-specific flavonoids in the cocoons. We propose a simple analytical method using a UV-VIS photospectrometer to roughly estimate the amount and composition of cocoon flavonoids. This may help in the genetic study and breeding of Ryokuken and Sasamayu races.

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© 2014 by The Japanese Society of Silk Science and Technology
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