An improved process for stably preparing of Antheraea pernyi fibroin aqueous solution

Abstract

A stable and reproducible preparation of A. pernyi fibroin (APF) aqueous solution is an important theme for using APF for medical applications. We experienced gelation and aggregation of APF during its preparation when following reported literatures. We reconsidered the whole preparation process to prepare AFP aqueous solution. The pretreatment and degumming process of the cocoons didn’t have influence. More than 15 M LiSCN was required for complete dissolution. The dialysis time was sensitive on gelation and shorter time was an improvement for the aqueous solution stability. Finally, addition of small amount of Ca²⁺ was effective to improve stability of the aqueous solution.