Using qPCR method to find the number of transgenes inserted in the Bombyx mori genome

Abstract

　　This report describes a method to ascertain the copy-number of transgenes of transgenic silkworm. For this study, probe-based quantitative polymerase chain reaction (probe qPCR) assay using comparative Ct method (ΔΔCt method) was used to detect the piggyBac sequence introduced into the silkworm genome. Using hsp90 gene as a reference, 0–8 copies of the piggyBac gene inserted into the silkworm genome were detected quantitatively.