The PCR Monitoring System Using Chemical Modified Primers

Abstract

Polymerase chain reaction (PCR) is the most convenient and direct method for analyzing and manipulating DNA and RNA. PCR needs polymerase, templates, and primers. In this review, we focus our attention on the chemistry of PCR primers. Because PCR is a basic technology in biology research fields, we sometimes use chemically labeled primers without any awareness of the chemistry they leave behind. In this primer designing, there are a lot of potential for different chemistry ideas and much study is still necessary to advance PCR for single-nucleotide polymorphism (SNP) typing, genetic diagnosis, and other fields. We provide an overview of the labeling chemistry of PCR primers, especially the concept and its application to SNP and gene typing, and show a new type of PCR monitoring system in here.