Abstract
Saliva, contains an abundance of proteins, offers an easy, safe, and non-invasive approach, and then possesses a high potential to investigate the stress. Alpha-amylase and chromogranin A are well known proteins as stress markers in saliva, however, past studies were limited to the effect against acute stress. Little is known about the effect of relaxing treatment after long-term stress. In this report, we examined effects of walking on Seaside therapy. We measured salivary amylase activities, blood pressures, and psychological stress response scale tests of about 40 volunteers. Using saliva samples showing a decrease in the activity of amylase after walking, salivary proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Western-blotting analysis was performed after SDS-PAGE, two major bands of alpha-amylase were shown in any cases. Dot-blotting analysis showed that the amount of the proteins cross-linked with antibody of alpha-amylase was increased in spite of the decrease in the amylase activity. The decrease in the amylase activity could not be caused by the decrease of the secretion to saliva. The activity of the salivary amylase might be controlled by other factors. Now, we examined the further analysis about other factors to elucidate the biomarkers in non-stress.
