The Effect of Roxithromycin on Chemotaxis of Th1, Th2 and Regulatory T Cells

Abstract

In this study, we investigated the affects of roxithromycin (RXM) on chemokine receptor expression and chemotaxis of Th1, Th2 and regulatory T cells (Treg) using FACSCalibur^TM and EZ-TAXIScan^TM. After culturing these cells with 10mM RXM contained RPMI medium for 18 hours, the expression of chemokine receptors (CXCR3 and CCR4) and chemotaxis were studied by FACSCalibur^TM and EZ-TAXIScan^TM. Flowcytometric analysis showed no significant difference of chemokine receptor-mean intensity (M.I.) on these T cells between control group and RXM-treated group. EZ-TAXIScan^TM revealed that 10mM RXM inhibited chemotaxis of Th1 and Th2 cells against IP-10 and TARC, respectively compared with control group. Taken these results, RXM may inhibit chemotaxis against IP-10 and TARC directly on both Th1 and Th2 cells without suppression of chemokine receptor (CXCR3 and CCR4) expressions. On the other hand, RXM has no affect of chemotaxis and chemokine receptor expression on Treg cells.Skin Research, Suppl. 19: 16-21, 2012