Abstract
Human papillomavirus (HPV) is an important etiological agent in different kinds of malignant and benign skin tumor, including squamous cell carcinoma and Bowen's disease. We report a case of multifocal vulvar Bowen's disease of human papillomavirus (HPV) type 16. A 62-year-old man presented with hypopigmentation with erythema and black to brown papules involving the perianal to right genitocrural region. In 2006, the patient had been diagnosed with Bowen's disease and subsequently undergone 3 surgical treatments without histopathological complete resection. Therefore, he had been introduced to our hospital and received a curative extensive resection of the skin and anorectal mucosa in 2011. The diagnosis was confirmed as multiple Bowen's disease. To check for HPV infection, PCR amplification and DNA sequencing were performed on extracted tissue DNA using GP5/GP6 primers, resulting in the detection of type 16 DNA among malignant types of HPV. Sequentially, amplification of papillomavirus oncogene transcript (APOT) assay showed only episome-derived HPV16 transcripts without integration into tumor DNA. Since previous reports showed that genome integration of malignant-type HPV such as type 16 was associated with the development of SCC, APOT assay may be valuable to discriminate SCC from non-invasive Bowen's disease.Skin Research, 13: 263-268, 2014
