Abstract
A method of “chondroitinase digestion and disaccharide mapping by paper chromatograpy” originally reported by Suzuki et al, and the electrophoresis in the cellulose acetate strip were applied in various human skin specimens in order to measure and identify the involved acid mucopolysaccharide (AMPS) in tissue. The present paper concerns AMPS constituents in normal skins, scars and keloids at every clinical stages.
The following results were obtained:
1) In normal skins, main AMPS constituents were found to be hyaluronate (HA), 61.4%and chondroitin sulfate (ChS) B, 19.8%, while ChS A and C were scarcely found.A small amount of chondroitin-like AMPS was also observed. Compared with the adult skin, human embryonic skin was rich in AMPS, especially in HA and ChS C
2) It was observed that contents of total AMPS and Chs B in scars were highly observed reflecting their developmental periods and clinical courses. The amount of total AMPS was found to be increased in around a year after onset and tended to be decreased gradually year by year. In hypertrophic stages, total AMPS contents was highest and high percentages of HA, ChS A and C were noted. In sclerosing stages, total AMPS contents dropped, while a family of ChS, especially ChS B rate rose up. In atrophic stages, the amount of total AMPS was much less, and high constant level of ChS rate (mainly ChS B) was recognized.
3) In keloidal tissues, total AMPS was determined in high amount, and it was equal to those of hypertrophic scars. HA rate was found to be approx. 30%, which was less than that of normal adult skin but larger than that of hypertrophic scars. ChS contents were also in high level, in which the rate of ChS A and C was shown succesively higher in early stage and then ChSB rate increased later.
4) Heparitin sulfate was found at approx. 20% of ChS B amount in skin of keloids and scars.
