Abstract
The authors investigated a method for the quantitative induction and challenge of the allergenicity of externally applied toiletories and cosmetics, including their components.
The experiment used oil-soluble cinnamic aldehyde and water-soluble formalin as allergens and guinea pigs as the experimental animals. As a result, a high sensiti&ation method was obtained, wich is carried out as follows: A 24-hr close patch is attached to the skin every other day over two weeks (a total of four applications). FCA is intradermally administered just before the 3rd application of the patch. The challenge step is performed by directly applying the test material.
This method was compared with other allergenicity evaluation methods. As a result, this method was found to be in no way inferior in sensitization performance to the others.
