Abstract
Epidermal cyst contents in saline solution were injected into the back skin of BALB/cAnNCrj mice and the areas of skin injected were examined histologically. The following results were obtained.
1) Epithelioid cell granuloma (EG) was developed to appear 28 days after subcutaneous injection of 10% epidermal cyst content in physiological saline solution into non-sensitized mouse with the content.
2) In two groups of content-sensitized mice with subcutaneous injections of O.5% epidermal cyst content and the supernatant of 10% content in physiological saline solution, respectively, EG was developed to appear 7 days after the injection in the former group only.
3) In two groups of non-sensitized mice with subcutaneous injections of O.5% content and the supernatant, respectively, none of the mice showed development of EG.
Basing on these findings obtained in the present experiment, it would be suggested that the injection of a whole amount of content of epidermal cyst and the sensitization in the experimental animals, with the content might be related to the development of EG. When non-sensitized mice were injected with a large amount of content of epidermal cyst, the animals were subsequently sensitized with the injected content in a certain period of time, resulting into development of EG.
