Abstract
The dissociated culture is a suitable system to observe behavior of functional molecules and an intercellular interaction in detail. The cultured neuronal network was confirmed to have a mechanism to modify own functional structure automatically. We found characteristic transient high frequency burst activity(HFB) during culture days. The neuronal network generated such particular spontaneous activity, suggesting that the HFB autonomously constructed a heterogeneous network structure suitable for neurotransmission. After the HFB, some neurons became to have many inputs while others became to have little. We confirmed that the HFB terminated within 10days, without a drastic change of the number of neurons. It suggests that the termination of the HFB activity was not caused by the decrease of the neurons, but was performed by the modification of the intercellular interaction.
