Abstract
In this paper we describe simple culture methods for barnacle larvae that do not involve changing the culture seawater during rearing to the cyprid stage. Larvae of the barnacles Amphibalanus amphitrite and Fistulobalanus albicostatus were reared under three different conditions: 1, the culture seawater was changed every day; 2, the culture seawater was not changed, but modified Provasoli Erd-Schreiber medium for diatoms was added to it; and 3, the culture seawater was not changed but the diatom medium and antibiotics (penicillin G and streptomycin) were added to it. Larvae of both barnacle species cultured without changing the seawater grew sufficiently well and metamorphosed into cyprids. Using the same method, cyprids of Megabalanus rosa were reliably produced. Therefore, a maintenance-free culture method that adds diatom medium and antibiotics into the rearing water can be effective in producing large amounts of cyprids.
