Serological diagnosis of antisalivary duct antibody (ASDA) and antinuclear antibody (ANA) by human salivary duct (HSD) cells in Sjögren's syndrome (SjS)

Abstract

This study was designed to evaluate the serological diagnosis of antisali vary duct antibody (ASDA) and antinuclear antibody (ANA) in sera of patients with Sjögren's syndrome (SjS) using human salivary duct (HSD) cells transformed by SV4O in vitro
The detection of ASDA and ANA in 52 sera from patients with SiS were examin ed by indirect immunofluorescent technique using HSD cells as substrate and the re sults were summarized as follows;
1) ASDA could be detected in 78.3% of sera from patients with definite SjS, but not in sera of patients with probable SjS and control.
2) Correlation between ASDA positive and lymphocytic sialoadenitis of definite SjS patients was recognized clearly.
3) ANA was found in 87.0% of sera from patients with definite SjS and 4.8% of sera from patients with probable SjS.
4) Three staining types of speckled, homogeneous and peripheral patterns could be classified in FITC positive nuclear and the speckled staining pattern was shown particularly in 73.9% of sera from patients with definite SjS.
These results indicated that HSD cells were very useful for serological diagnosis of SjS