Abstract
Angiogenesis plays a critical role in the growth and metastasis of solid tumors. Vascular endothelial growth factor (VEGF) is one of the most potent angiogenic fac torsproduced by tumor cells, and its synthesis has been shown to be modulated through the activation of some transcription factors including HIF-1 induced by hypoxic condition. We transfected synthetic oligodeoxynucleo tides (ODNs) with consensus sequence for HIF-1 bind ing (HIF-1 decoy ODNs) into cultured cancer cells (SAS cells) using the hemagglutinating virus of Japan (HVJ)-liposome method. The hypoxia induced overexpression of VEGF in SAS cells could be apparently suppressed by the transfection of HIF-1 decoy ODNs. These results suggest that the HIF-1 decoy strategy would be effective for regulating tumor growth by reducing angiogenic ac tivityof cancer cells through HIF-1-mediated gene trans activations.
