The role of NF-κB in the expression of IL-8 gene from human adenoidal fibroblasts

Abstract

It has been suggested that inflammatory cytokines play an important role in the crisis and prolongadon of otitis media with effusion. NF-κB is a transcriptional factor that regulates the gene expressions of inflammatory cytokines. The aim of this study is to invesdgate the role of NF-κB activadon in the gene expression of inflammatory cytokines in cultured adenoidal fibroblasts.
The acdvadon of NF-κB was evaluated by electrophoretic mobility shift assay (EMSA). Furthermore, IL-8 gene expression was examined by RT.PCR and ELISA.
EMSA revealed that NF-κB was acdvated obviously by the sdmuladon with IL-1β in adenoidal fibroblasts. The expression of IL-8 was suppressed in a dose-dependent manner when the acdvation of NF-κB was inhibited by TPCK. These results suggested that the activation of NF-κB would be essendal for the expression of IL-8 in adenoidal fibroblasts.