Abstract
Gemcitabine (GEM) is the most commonly used chemotherapeutic agent for pancreatic cancer, but the molecular mechanisms of the sensitivity and/or resistance to this agent are not yet fully understood. In this study we used microarray analysis (Affymetric Human Genome U133 Plus 2.0 Array) to examine the influence of GEM in a pancreatic cancer cell line (PANC-1). Three hundred seventy two genes whose expressions were significantly altered were identified. Using Ingenuity Pathways Analysis (IPA), the gene cluster relating to gene expression, cell death, DNA replication, and cell cycle has been especially changed. Three important networks were mapped and two important genes (p53 and myc) were identified. The expression level of TP53INP1 mRNA which is related to p53 was measured by semi-quantitative RT-PCR analysis. The TP53INP1 expression was increased by GEM in a dose dependent manner. These data suggest that p53 and myc are critically involved in the action of GEM in a pancreatic cancer cell line.
