2023 Volume 38 Issue 1 Pages 44-50
Conventional histopathological evaluation can only view arbitrary cross sections of a histologic specimen. However, recently, it is possible to observe the entire specimen using tissue clearing technology with three-dimensional imaging. If the entire specimen can be evaluated, the three-dimensional structure of the tissue is observed. As a result, there are benefits such as fewer missed lesions and the clarification of new pathologic entities. There are dozens of tissue clearing reagents available. Each reagent and microscope has its own characteristics. It is important to select the appropriate reagent and microscope according to the intended purpose. Tissue clearing technology is reviewed in this paper. The evaluation of Pancreatic Neuroendocrine Neoplasms by tissue clearing technology with three-dimensional imaging is introduced.
