Abstract
The aim of this study was to investigate the effect of A-3922, a dihydrobenzofuran derivative, on hydroperoxide (H2O2) -induced PC12 cell injury. H2O2-induced PC12 cell injury was determined by lactate dehydrogenase (LDH) release. The concentration of lipid peroxide was determined from the amount of thiobarbituric acid reactive substances (TBARS) in PC12 cell homogenate. Following exposure to 150 μM H2O2 for 45 min (Condition I), the LDH activity increased to 181 ± 6 % of control activity (n = 11, P < 0.01) . The LDH activity significantly decreased to 128 ± 6 % (n = 15, P < 0.01 vs Condition I) when PC12 cells were exposed to 150 μM H2O2 combined with 10 μM A-3922 (Condition II), and 161 ± 7 % (n = 15, P < 0.01 vs Condition I) when pre-treated with 10 μM A-3922 prior to the addition of 150 μM H2O2 (Condition III) . Lipid peroxidation of PC12 cells induced by 25 mM 2, 2'-azobis (2, 4-dimethyl-valeronitrile), a lipophilic radical initiator, was inhibited by the addition of 100 μM A-3922. These results indicate that A-3922 exerts a protective effect against oxidative stress-induced PC12 cell injury, in part through the inhibition of lipid peroxidation in the cell membrane.
