Abstract
Resting cells of a marine Vibrio sp. were exposed to sodium cholate of up to 0.1% for 30 min at higher salt stresses, and surviving cells were counted. As a result, the number of surviving cells drastically decreased to 103.9 CFU ml−1 at 1.40 M NaCl from 108.6 CFU ml−1 at 0.50 M NaCl. No surviving cell was observed at 1.85 M NaCl, where structural damage to the cells was confirmed by means of scanning electron micrographs. Viable cells did not decrease at all due to exposure to 1.85M NaCl alone. These results indicate that the intact structure of cell wall plays an important role in preventing the influx of drugs into periplasm for marine Vibrios.
