Abstract
A bioreactor packed with halo-tolerant microorganisms was investigated for the treatment of waste decomposing biological materials on a laboratory scale. The waste of sea water after complete decomposition of jellyfish by means of protease E77 were used as a model solution for this purpose. The waste mentioned above contained CODMn from 1,040 to 3,440 mg/L. Masses of halo-tolerant microorganisms were isolated from the enriched cultural solution including the waste, because these microorganisms utilized the waste as a source of substrates. The harvested microorganisms were encapsulated in the immobilized 3% agar pellets. A bioreactor packed with the pellets including microorganisms was circulated aerobically by the waste of sea water after the decomposing jellyfish at 32°C. 84% of CODMn was removed from the original value after the packed bioreactor was circulated over 4 days. Combined with coagulation-sedi-mentation and treatment of activated carbon adsorption, the CODMn content became almost zero. The yeast using jellyfish wastewater as a nutrient source was identified as Rhodotorula mucilaginosa by means of 28S rDNA-D1/D2 analysis. We determined six bands for bac-teria from the polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) on the basis of 16S rDNA.
