Synthesiology
Online ISSN : 1882-7365
Print ISSN : 1882-6229
ISSN-L : 1882-6229
Research papers
Development of an accurate and cost-effective quantitative detection method for specific gene sequences
- Development of a quantitative detection method for specific gene sequences using fluorescence quenching phenomenon -
Naohiro NODA
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JOURNAL FREE ACCESS

2010 Volume 3 Issue 2 Pages 147-157

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Abstract
DNA and RNA quantifications are essential in various fields such as biomedicine, agriculture, fishery, environment, and food. We have developed an accurate and cost-effective method for the quantification of specific nucleic acid sequences; the method involves the use of the fluorescent quenching phenomenon via an electron transfer between the dye and a guanine base at a particular position. This paper describes the elemental key technologies and their synthesis for the development of such a gene quantification method. Furthermore, based on the findings of a collaborative research project with a private company, we report the scenario for the industrialization and the practical use of the developed method.
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© 2010 National Institute of Advanced Industrial Science and Technology(AIST)
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