Effect of Ozone on Glutathione in Vivo
1975 Volume 10 Issue 2 Pages 58-62
Details
1975 Volume 10 Issue 2 Pages 58-62
Ozone effect on the glutathione (GSH) content of mouse lung was studied.
In acute experiments mice were exposed to various concentrations of O3 ranging from 1.2 to 8.2 ppm for 4 hours. There was a significant decrease in GSH content immediatly after the exposure to 8.2 ppm O3; however, there was no changes in GSH content of mouse exposed to below 4.0 ppm O3. Two days after the exposure to 4.0 ppm O3, GSH content increased, and the level of the GSH content lasted for several days.
In chronic experiments mice were expoed to 0.2, 0.5 and 1.0 ppm of O3 4 hours daily for 30 days, respectively. There was a progressive increase in GSH content according to the concentrations of O3 and period of exposure. In addition, the activities of peroxidative metabolic pathway enzymes (glucose-6-phosphate dehydrogenase, glutathione reductase and glutathione peroxidase) increased in lungs of mice exposed repeatedly. Those mice exposed repeatedly for 7 days were found to be resistant to subsequent challange with lethal doses of O3.
These results suggest that the enzyme activites of peroxidative metabolic pathway as well as GSH levels in lungs are usable as sensitive biochemical indicators of chronic effects of low concentrations of O3.
