Abstract
Effective method for extracting mutagens in engine oils was investigated in order to determination emission level of mutagens from gasoline and diesel cars, mutagenic activity in engine oil was attended and a effective method for extracting chemicals was investigated. Engine oils were collected from 12 gasoline which were driving in Tokyo, and 7 diesel cars which were driving outside of Tokyo. Engine oils from mileage were also collected for every 1, 000 km after filling with new oils. The pre-incubation modified method of Ames original method was used for estimating mutagenicity using Saimonella typhimurium strains TA100 and TA98 with and without S9mix.
The combination of the reflux methanol extraction and liquid-liquid extraction using isopentane and acetonitrile was effective for detecting mutagens in both gasoline and diesel engine oils. Relationship between the mutagenicity and the mileage was observed towards both types of cars. Mutagenic activities of gasoline engine oils showed generally higher values than those of diesel engine oils. Mutagenicities of gasoline engine oils from longer distance driving cars more than 30, 000km were also higher values than shoter distance driving cars.
