Abstract
A large amount of in jection-high performance liquid chromatography (HPLC) with spectrofluorometric detection was studied for analysis of the polynuclear aromatic hydrocarbons (PAHs) in trace suspended particle matters (SPM) collected by SPM monitor every 1 hour.
The procedures of this method were as follows: the sample solution was prepared to same composition of mobile phase (acetonitrile-water mixture, 80: 20, v/v) and then a large amount (usually 200μl) of sample solution was injected to HPLC. It was proved that this method could determine accurately and sensitively the 3 PAHs, benzo (a) pyrene (Ba P), benzo (k) fluoranthene (Bk F) and benzo (ghi) perylene (Bghi P).
The detection limits of PAHs by this analytical procedures in the case of 200μl in jection are 0. 063 ng/ml (absolute amount: 0.013ng) for Ba P, 0.072ng/ml (0.014ng) for Bk F and 0.71ng/ml (0.14ng) for Bghi P, respectively and the reproducibility of 3 PAHs are within 1.1% as expressed in terms of the relative standard deviation.
This method has been performed to prove the effectiveness of the trace analysis of 3 PAHs in SPM samples collected every 1 hour by SPM monitor utilized β-rays absorption method (suction rate: 18l/min, sampling time: 55min. 32 sec.).
