1994 Volume 29 Issue 2 Pages 92-103
Enzyme fluorometry using p-hydroxyphenylacetic acid and peroxidase has become a well-established continuous monitoring method for hydrogen peroxide, and provided important field data mainly in North America and Europe. We have built an automatic analyzer based on the method for continuous field measurements at remote sites and on board of research vessels that was successfully used during several compaigns. The system consists of air scrubbers, multichannel flow system and dual channel fluorometer. This technical note describes the principle, construction, installation, operation, calibration and maintenance of the enzyme fluorometric hydrogen peroxide analyzer. Emphasis is placed on how to avoid troubles with the sensitive instrument to help disseminate the method, and obtain a larger data base about peroxides in East Asia.
