Characteristics of Laser-Induced Fluorescence Spectra Changes in Peanut Leaves by Ozone Exposure and Ultraviolet Irradiation
2004 Volume 39 Issue 2 Pages 89-96
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2004 Volume 39 Issue 2 Pages 89-96
Laser-Induced Fluorescence (LIF) spectra from peanut leaves excited by UV laser were measured. The LIF spectra were changed by both the ozone exposure and the ultraviolet irradiation from UV-B fluorescent lamp immediately. The characteristics of the LIF spectra changes into the ozone and the ultraviolet stresses were quantified by the fluorescence ratios F685/F530 (red/green) and F740/F685 (far-red/red).
For the ozone exposure, F685/F530 ratio decreased in proportion to the logarithm of the exposure time as well as in proportion to the ozone concentration. F740/F685 ratio increased during the first hour then decreased gradually. For the ultraviolet irradiation, F685/F530 ratio decreased in proportion to the ultraviolet fluence, while F740/F685 ratio increased in proportion to the irradiation time. By clarifying the characteristics of LIF spectra changes in plant leaves, it is thought that the early detection of any damage and stress factor specifics becomes possible.
