A study of isolated organisms in bile directly collected by endoscopic cannulation of the papilla of Vater

Abstract

Bile collections were attempted in 63 patients,40 males and 23 females, after endoscopic cannulation of the papilla of Vater at the time of endoscopic retrograde cholangiography (ERG)or/and endoscopic retrograde biliary drainage (ERBD) for the purpose of examining organisms in bile.82 collections were successful in 63 patients. The materials were injected into the CO₂ filled bottle and cultured by aerobic and anaerobic method. S. faecalis was the commonest isolate and account for 34.1%. But this was suspected to be one of contaminants. As Gram negative rods, P. aeruginosa, Klebsiella and E. coli were detected and respectively account for 29.4%,13.4% and 9.8%. P. aeruginosa was also possible to be a contaminant. As anaerobic species, Clostridium perfringens was isolated from two out of 82 cases and revealed mixed infection with Gram negative rods, as frequently encountered.