Abstract
was performed. The acid colloid precipitated by glycol chitosan (GCh) was presumed to be sulfated glycoprotein-like substances, which were examined with ultrafiltration technique (fraction molecular weight 200,000) and infra-red spectroscopy.
The degree of the decrease in OD 440 nm with addition of GCh, that is, as content of acid colloid in bile, was studied in gallstone biles of 58 cases and in control biles of 5 cases.
In gallstone biles the acid colloid content was the highest in calcium bilirubinate stone cases and was not almost detected in black stone cases. And the acid colloid in infected biles was significantly higher than that in aseptic biles.
The acid colloid in bile, which is supposed to be responsible for gallstone formation, was indirectly identified by simple applicaion of colloid chemistry.
