Abstract
We made a detailed assessment about the influence of bile acids for the concentration of ionized calcium in human gallbladder bile.
In Ca-bile acid solution, calcium binding capacity of glycine-conjugated bile acids and dihydroxy bile acids were respectively dominant in comparison with that of taurine-conjugated ones and trihydroxy ones. Furthermore, the ionized calcium concentration decreased on adding lecitin. These facts show that the calcium binding capacity of bile acids may be affected according to the bile acid micelle size.
Addition of various bile a cids also reduced ionized calcium concentration in bile, and the agreement with the results of our experiments in Ca-bile acid solution. Ionized calcium concentrations in human bile with black stones or calcium bilirubinate stones were significantly hygher than that in the bile without gallstones.
It is suggested that ionized calcium, rich in reactivity, is quite requisite in the pathogenesis of pigment gallstones.
