PERSONAL IDENTIFICATION BY DNA ANALYSIS OF SAMPLES FROM DENTAL PROSTHESES MADE OF ACRYLIC RESIN

Abstract

The procedures and possibilities for personal identification by DNA analysis of samples from dental prostheses made of acrylic resin were investigated. Acrylic resin pieces dipped in whole saliva for a moment, dried in air and left for at least two months at room temperature retained saliva stains that could be used as materials for DNA analysis for personal identification. The amounts of DNA extracted from 0.5×0.5×0.1cm resin pieces prepared from 55 resin prostheses used by the patients ranged from 35.7ng to 1.52μg and were significantly different among the samples. The amounts of extractable DNA did not correlate with the period of time the prostheses were left at room temperature or the period of time they were used in the oral cavity. Sex determination by amplification of segments of the amelogenin gene and typing of the 184bp fragment in the D4S43 locus was possible from all of the extracted DNA. Resin prostheses used in the oral cavity and left at room temperature for as long as approximately 200 days could be used for DNA extraction followed by DNA analysis. The present results provide a procedure that can be used from sampling of DNA from resin prostheses used in the oral cavity to typing by DNA analysis.