2002 Volume 18 Issue 2 Pages 65-73
Oxidative stress influences the pathologic development of many diseases. Vascular endothelial cells are particularly vulnerable targets for reactive oxygen. In this report, we examine the physiological changes, cell viability, induction of HSP 70 and the clotting and fibrinolytic factors of vascular endothelial cells under oxidative stress from hydrogen peroxide. Under such stress from 0.0003% to 0.003% hydrogen peroxide, the expression of HSP 70 increased 2.5-fold compared with that of the control. The production of PAI-1 of vascular endothelial cells tended to increase up to 0.003% hydrogen peroxide. The cell viability and tPA production of vascular endothelial cells decreased only once (00.001%), but then held steady (0.0010.003%). When oxidative stress exceeded 0.003% hydrogen peroxide, the induction of HSP 70, cell viability, and the tPA and PAI-1 production of vascular endothelial cells decreased.
Thus, under oxidative stress, vascular endothelial cells virtually always expressed HSP 70 in response, and would be cytoprotected by induced HSP 70, and the blood clotting and fibrinolytic systems depended on coagulation. Under the excessive oxidative stress (over 0.003% hydrogen peroxide), all of them (cell viability of HUVEC, production of tPA and PAI-1 and expression of HSP 70) decreased.
