Abstract
Some eggs are frozen for preservation and are thawed when they are used in microinsemination process such as ICSI. However, the elasticity of egg makes the manipulation difficult to perform. It may be possible to simplify the process by performing the manipulation for frozen egg, because there is no need to concern about the elasticity when eggs are in frozen state. Therefore, we propose a new type of microinsemination system, which is, a small hole is opened on a frozen egg by drilling and a sperm is injected into it. We developed a new system to establish this process. The ultimate objective of this system is to establish the microinsemination system for frozen egg of mouse. In the experiment we used immature eggs of a Silver whiting at first and then proceeded to the experiment of frozen mouse egg and succeeded to open a hole on it.
