Abstract
Phospholipase A [EC 3.1.1.4] was purified from the venom of Habu (Trimeresurus flavoviridis) by the gel filtration on Sephadex G-100 and the column chromatographies on CM-cellulose, with a 3.7% over-all yield and a 7.1-fold increase in the specific activity. The purified enzyme was proved to be homogeneous by the disc electrophoresis. And the gel filtration on a Sepharose 6B column of the purified enzyme indicated a molecular weight of arround 26, 000. The purified enzyme was caused immediate loss of the activity by the addition of EDTA. But the activity was recovered by the addition of excess CaC12 in the presence of EDTA and no activity was recovered by the addition of Mg++, Cd++ and Zn++, respectively, in the place of Ca++.
