THE GELATIN PARTICLE INDIRECT AGGLUTINATION TEST, A MEANS OF SIMPLE AND SENSITIVE SERODIAGNOSIS OF CHAGAS' DISEASE

TAKAO YAMASHITA; HISAMI WATANABE; MARISEL MALDONADO; MARIA ANGELICA LEGUIZAMON; TADASHI WATANABE; SUSUMU SAITO; TAKESHI SHOZAWA; YOSHIYA SATO; FUJIRO SENDO

doi:10.2149/tmh1973.22.5

TAKAO YAMASHITA, HISAMI WATANABE, MARISEL MALDONADO, MARIA ANGELICA LEGUIZAMON, TADASHI WATANABE, SUSUMU SAITO, TAKESHI SHOZAWA, YOSHIYA SATO, FUJIRO SENDO

Author information

TAKAO YAMASHITA
Department of Immunology and Parasitology, Yamagata University School of Medicine
HISAMI WATANABE
Department of Medical Zoology, Niigata University School of Medicine
MARISEL MALDONADO
Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncion
MARIA ANGELICA LEGUIZAMON
Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncion
TADASHI WATANABE
Department of Immunology and Parasitology, Yamagata University School of Medicine
SUSUMU SAITO
Department of Immunology and Parasitology, Yamagata University School of Medicine
TAKESHI SHOZAWA
Japan Medical Cooperation project on Chagas' disease supported by Japan International Cooperation Agency (JICA)
YOSHIYA SATO
Department of Parasitology, and Research Center of Comprehensive Medicine, School of Medicine, University of Ryukyus
FUJIRO SENDO
Department of Immunology and Parasitology, Yamagata University School of Medicine

JOURNAL FREE ACCESS

1994 Volume 22 Issue 1 Pages 5-8

DOI https://doi.org/10.2149/tmh1973.22.5

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Abstract

An indirect agglutination test using a Trypanosoma cruzi antigen-coated gelatin particles was employed to diagnose trypanosomiasis in Paraguay. Results with this test were quite comparable to those obtained with enzyme-linked immunosorbent assay (ELISA). Furthermore, nonspecific reaction to the gelatin particles alone was not found in either acute or chronic infection. This method is more convenient than the ELISA, since the antigen-conjugated particles is stable for at least 1 year at 4°C and since the test itself is short and simple to perform and does not require specialized equipment.

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