Abstract
It was attempted to cultivate Trypanosoma garnbiense (Wellcome strain), maintained by mouse-passages for years, in coexistence with the mouse L-cells in vitro at 37 C. In the system of culture the population of the cultured trypanosomes increased 3-fold in 24 hours, then decreased. The fact that the doubling time of trypanosomes was longer in the culture system than in an infected mouse seemed to be related to the suppressed division of parasites. It was observed that the final stage of parasite division, that is, the cytoplasmic separation into 2 individuals was much prolonged in culture, although the kinetoplastic, nucleal and flagellar duplication was carried through without suppression. In the control without the L-cells, no multiplication of trypanosomes took place. Infectivity of the cultured trypanosomes to mice did not change throughout the course of culture, at least until the time of the maximum growth of parasites. The subculture of trypanosomes was not successful.
