Abstract
The agarose plate method using dog neutrophils and human monocytes as migrating indicator cells were used to demonstrate neutrophil chemotactic factor and monocyte chemotactic factor, respectively, in dogs infected with Babesia gibsoni. The experiments were done in the supernatant from in vitro cultures of spleen cells, peripheral blood lymphocytes as well as extracts of spleen cells.
In particular, it was suitable to use canine neutrophils as migrating indicator cells for demonstrating neutrophil chemotactic factor in 2.5 × 105 cells per well of normal canine leukocytes incubated for 2 hours in agarose plate with 10% inactivated normal canine serum. Supernatants from in vitro cultures of canine spleen cells (3 weeks postinfection) and peripheral blood lymphocytes (3 days postinfection) showed an increased chemotactic activity of neutrophils as compared with that of normal dogs. Similarly, supernatants from in vitro cultures of canine spleen cells (2, 3 and 25 weeks postinfection) and peripheral blood lymphocytes (7 days postinfection) showed a tendency to contain monocyte chemotactic activity.
