Abstract
We propose the non-label and invasive analysis method for drug transport using an oocyte-based field effect device, which is based on potentiometric detection of extracellular potential change derived from exchange of molecular charges at cell membrane/gate insulator. The extracellular potential change at cell membrane/gate insulator can be directly transduced into electrical signal such as threshold voltage changes using the oocyte-based FET. The time course of threshold voltage change can be monitored during the uptake of common substrate through the transporter at the cell membrane without any labeling materials. The platform based on the oocyte-based FET is suitable for a simple and convenient system for drug screening in pharmaceutical lead discovery.
