Abstract
To develop a highly sensitive immunoassay, both enhancement of specific signals and reduction of nonspecific signals should be achieved. In this study, we investigated a novel biointerface for highly sensitive immunoassays by integrating a phospholipid polymer with a nanoscale surface modification process known as the electrospray deposition (ESD) method. The surface prepared by ESD has a nanosphere-shaped polymer structure and therefore the specific signals from analytes in the enzyme immunoassay were significantly enhanced due to an increase in the surface area. The sprayed phospholipid polymer prevented nonspecific protein adsorption effectively lowering the nonspecific signals which cause the high background or noise level. Furthermore, the phospholipid polymer has active ester groups for conjugation of antibodies, and the stability of the antibodies conjugated to the polymer surface was improved drastically. The nanosphere-shaped phospholipid polymer surface can be used to yield a highly sensitive, stable, and reliable assay.
