Abstract
To construct polymer hydrogel microstructure containing biomolecules as a biosensor in microfluidic devices by a conventional photoirradiation, new photocrosslinkable polymers composed of 4-(4methoxycinnamoyl)phenyl methacrylate and 2-methacryloyloxyethyl phosphorylcholine (PMMC) was synthesized. The polymers were used as prepolymers to make hydrogels by photoirradiation. Moreover, PMMC gel exhibited excellent performance of protein adsorption resistance compared with Poly(vinyl alcohol)-N-methyl-4(4'-formylstyryl) pyridinium methosulfate acetal (PVA-SbQ), and succeeded in the immobilizing of enzyme. In the future, the immobilized enzyme will be checked in detail for application of PMMC to a biosensor. PMMC is thought to be increasing the permeability of the biomolecules to the inside of the gel compared with PVA-SbQ which is the material commonly used in the current researches. From the results so far, it is expected that PMMC can be used for simple fabrication of enzyme immobilizing membranes for highly sensitive biosensors.
