2007 Volume 32 Issue 4 Pages 929-932
We performed H+, H2+ and He+ ion-beam irradiation into biodegradable polymer sheets of Poly-L-lactic acid (PLLA) at an energy of 150 keV with a fluence of 1 x 10^15 ions/cm^2. The irradiated surface was investigated by means of FT-IR-ATR, Raman spectroscopy, and a contact angle meter. Ion-beam irradiated layer was exfoliated from the substrate in a water solution, and thin films were obtained. Next, H+, H2+ and He+ ion-beam irradiation was performed at an energy of 150 keV with a fluence of 1 x10^15 ions/cm^2 with a stainless-steel mask on the sample surface. We fixed the sheets to the bottom of a cell culture dish and seeded mouse fibroblast (L929) cells on the sample surface. These cells attached and spread on the micro-patterned domains, and the domains spontaneously exfoliated from the substrate. Consequently, micro-patterned cellular chips were obtained. These techniques and production methods are expected to apply to scaffolds, artificial organs and cell diagnosis tools.
