Adsorption of protein on three dimensional large pore cage type mesoporous material

Abstract

Highly ordered three dimensional large pore cage type mesoporous silica (KIT-6) has been synthesized using P123 and n-butanol as a structure directing agent and a co-solvent respectively and characterized by XRD and N2 adsorption. Adsorption of lysozyme on the above material has been studied as a model protein adsorption system and its adsorption isotherm has been fitted by Langmuir equation. The amount of lysozyme adsorbed over KIT-6 at the solution pH of 11, which is near the isoelectric point of lysozyme, is found to be 22.1 micro mol/. It has been found that the amount of lysozyme adsorbed over KIT-6 is much higher than that of MCM-48 which possesses three dimensional mesoporous structure with small mesopores.