Abstract
We developed a new solid biointerface for highly sensitive microchip immunoassay by integrating a phospholipid polymer (poly [2-methacryloyloxyethyl phosphorylcholine (MPC) -co- n-butyl methacrylate (BMA) –co- p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate (MEONP)]: PMBN) with a nanoscale surface modification process known as the electrospray deposition (ESD) method. The PMBN surface which can conjugate antibodies prevented the nonspecific protein adsorption. The PMBN surface coated by ESD has a nanosphere-shaped structure and showed a high water repellent property. A large amount of antibodies were immobilized using the PMBN surface coated by ESD with surfactant in the solution of primary antibodies. When surface tension of aqueous solution of antibodies was lowered, antibodies could bind to inner surface of PMBN nanostructure and arranged three-dimensionally. High density antibodies immobilized PMBN surface coated by ESD enhanced the specific signal and showed low nonspecific signal in the microchip immunoassay. The PMBN surface coated by ESD realized highly sensitive microchip immunoassay for detecting a small volume of sample in a short time.
