2009 Volume 34 Issue 1 Pages 1-8
Monolayer formation and behavior on a water surface of chromoionophore I (ETH 5294), a lipophilic isologue of oxazine-dye Nile Blue, were studied on the basis of surface pressure-molecular area (π-A) isotherms. Chromoionophore I formed a Langmuir-Blodgett film, and the stability of the monolayer appears to depend on the subphase pH as well as the degree of protonation in the chromoionophore I molecule. The chromoionophore I monolayers on the surface of subphase which was slightly basic showed favorite relaxation profiles, while it appeared to be hard and fragile on the acidic subphase. Adsorption experiment with ferritin has demonstrated the utility of chromoionophore I monolayer as a potential template although parameters which dictate the suitable conditions have to be further optimized to obtain a highly densely packed protein array.
