Abstract
To develop hybrid artificial organs, we prepared a heterogeneous functioned polymer membrane as an interface between blood and tissue. The membrane was constructed by three polymer layers, that is, cellulose acetate membrane as a base membrane, photo-reactive gelatin and a phospholipids polymer (poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)](PMB)). Gelatin and PMB layers were introduced on each side of the cellulose acetate membrane. The gelatin layer played as cell adhesive, but PMB layer prevented cell adhesion. We observed good cell proliferation on the gelatin side and blood compatibility on PMB layer. The heterogeneous functioned polymer membrane possessed permeability of insulin. The heterogeneity of this membrane represented the two conflicting surface properties in one: cytocompatible surface on one side and an antithrombogenic surface on the other side. We considered that the membrane has the solute permeability to transport the products of cells. It could be concluded that the membrane is excellent hybrid of polymer and cells for realizing hybrid artificial organs.
