Substrate specificities of wild- and mutated-type farnesyl diphosphate synthases with artificial substrate homologs

Masahiko Nagaki; Kazuhiko Miyata; Jun Kawakami; Ryo Miyamoto; Yuji Maki; Hiroshi Sagami; Tanetoshi Koyama

doi:10.14723/tmrsj.34.533

Masahiko Nagaki, Kazuhiko Miyata, Jun Kawakami, Ryo Miyamoto, Yuji Maki, Hiroshi Sagami, Tanetoshi Koyama

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Keywords: wild and mutated type of farnesyl diphosphate synthase, substrate specificity, homologs of isopentenyl diphosphate, epoxygeranyl diphosphate, epoxyfarnesol

JOURNAL FREE ACCESS

2009 Volume 34 Issue 3 Pages 533-536

DOI https://doi.org/10.14723/tmrsj.34.533

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Abstract

6,7-Epoxygeranyl diphosphate was examined for reactivity as an allylic substrate of wild and mutated farnesyl diphosphate (FPP) synthases of Bacillus stearothermophilus. 6,7-Epoxygeranyl diphosphate was accepted by wild and mutated (Y81R, Y81D, and Y81S) farnesyl diphosphate synthases as a substrate in reaction with isopentenyl diphosphate, yielding 10,11-epoxyfarnesyl and 14,15-epoxygeranylgeranyl diphosphates, respectively.

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